EVO视讯 EVO真人生命基因基于自主研发的EditX™基因编辑平台,采用优化升级的CRISPR/Cas9系统,设计科学的TSPO基因敲除方案。顺利获得RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HAP1细胞中,然后经过真核抗性标记筛选出阳性细胞池,再使用3D单细胞打印技术挑选单克隆细胞,最后顺利获得基因组测序验证,检测合格后对TSPO基因敲除的HAP1细胞进行扩增和冻存。
| 货号 | EDC08127 |
|---|---|
| 产品名称 | TSPO基因敲除HAP1细胞 |
| 物种 | 人 |
| 细胞 | HAP1 |
| 细胞别名 | HAP-1 |
| 消化时间 | 2 min |
| 基因 | TSPO |
| 传代比例 | 1:8~1:10 |
| 基因ID |
706
|
| 摘要 |
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | IMDM+10%FBS |
| 冻存培养基 | 90%FBS+10%DMSO |
* 仅供科研使用,不适用于人体或动物,包括临床、治疗或诊断用途。
* 研究用途免责声明:本内容基于公开的研究数据、生物信息学资源及计算分析生成,仅供研究参考。
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